IN SITU HYBRIDIZATION Protocol
Wilcox, J.N., Smith, K.S., Williams, L.T., Schwartz, S., and Gordon, D. Platelet-derived growth factor mRNA detection in human atherosclerotic plaques by in situ hybridization. J. Clin. Invest. 82, 1134-1143, 1988.
Wilcox, J.N., Smith, K.M., Schwartz, S.M., Gordon, D. Localization of tissue factor in the normal vessel wall and in the atherosclerotic plaque. Proc Natl. Acad.Sci. 86, 2839-2843, 1989.
Melton, D.A., Krieg, P.A., Rebagliati, M.R., and Maniatis, T., Zinn, K., Green, M.R. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucl. Acids Res. 12, 7035-7056, 1984.
CURRICULUM VITAE
JOSIAH N. WILCOX, Ph.D.
ADDRESS:
Emory University School of Medicine
Division of Hematology/Oncology
1639 Pierce Dr.; Rm 1115 WMRB
Atlanta, GA 30322
404-727-3628 (ph)/404-727-3404 (fax)
Email: medjnw@emory.edu
EDUCATION:
Ph.D. in Neuroscience 1977-1982
Rutgers University, Newark, N.J.
M.S. in Biology 1975-1977
Adelphi University, Garden City, N.Y.
B.S. in Biology 1969-1973
Long Island University, Southampton, N.Y.
PROFESSIONAL EXPERIENCE:
Associate Professor Oct 1990 - Present
Division of Hematology/Oncology
The Winship Cancer Institute
Emory University School of Medicine
Division of Cardiology
Department of Medicine
Emory University School of Medicine
Affiliate Scientist Sep 1991 - Present
Division of Pathobiology and Immunobiology
Yerkes Regional Primate Center
Assistant Professor of Pathology Oct 1991 - Present
Department of Pathology and Laboratory Medicine
Emory University School of Medicine
Research Scientist/Group Leader Jan 1987-Oct 1990
Department of Cardiovascular Research, Genentech, Inc.
Associate Research Scientist Jan 1986-Jan 1987
Department of Molecular Biology, Genentech, Inc.
Postdoctoral Fellow Dec 1982-Dec 1985
Center for Reproductive Sciences, Columbia University Physicians and Surgeons, New York, N.Y.
AWARDS AND HONORS:
1995 - Fellow of the Japan Society for the Promotion of Science
1987 - Tanaguchi Foundation Fellow, Kyoto, Japan
1986 - S. Weir Mitchell Award, Am. Acad. Neurology
PROFESSIONAL ACTIVITIES:
University Activities:
1994 Lecturing, IBS718 "Molecular Mech/Therapeutics of Cardiovascular Disease
1995-Present Member, Committee on Faculty Appointments and Promotions
1998-Present Member, University Research Committee
Manuscript review
Proceedings of the National Academy of Science (USA); American Journal of Pathology; Journal of Histochemistry and Cytochemistry; Circulation Research; Circulation; Journal of Clinical Investigation; Arteriosclerosis, Thrombosis and Vascular Biology; Journal of Vascular Research; American Journal of Physiology; Trends in Cardiovascular Medicine; Thrombosis Research; Laboratory and Clinical Medicine; Blood; Endocrinology; Life Sciences; Clinical Science; Journal of Experimental Medicine
Editorial Boards
1996-Present Associate Editor Journal of Histochemistry and Cytochemistry
Corporate Consulting
Bristol-Myers Squibb, Prism Pharmaceutical, COR Therapeutics, Glycomed, Henri Beaufour Institute USA, Ciby Geigy, Novo Nordisk, Berlex Biosciences, Devices for Vascular Intervention, Advanced Cardiovascular Systems, Smith Kline, Daiichi Pharmaceutical Co., Mitsubishi Chemical Corp., Amgen, Otsuka Pharmaceutical Co., Kowa Co.
Grant Review
1993 NIH, NHLBI Reverse site visit review committee
1994 NIH, NHLBI Parent committee Ischemic Heart SCOR proposals
1994 NIH, NHLBI Pathology A, Ad Hoc review committee
1995-97 AHA GA/SC Peer Review Board
1997 NIH, NHLBI Research Training Special Emphasis Panel
1997 NIH, NHLBI Pathology A review committee
1998 AHA Southern Research Consortium, Chairman Peer Review Committee #2
Vascular Wall Biology, Thrombosis, Lipoproteins and Lipid Metabolism
1998 NIH, NHLBI Ad Hoc Reviewer for Tissue Engineering, Biomimetics and Medical
Implant Science RFA
PUBLICATIONS:
Diakow, C., Wilcox, J.N., and Woltman, R. Female frog reproductive behavior elicited in the absence of the ovaries. Horm. Behav. 11, 183, 1978.
Wilcox, J.N. and Feder, H.H. Long-term priming with a low dosage of estradiol benzoate of an antiestrogen (Enclomiphene) increases nuclear progestin receptor levels in brain. Brain Res. 266, 243-251, 1983.
Wilcox, J.N., Barclay, S.R., and Feder, H.H. Administration of estradiol-17B in pulses to female guinea pigs: Self-priming effects of estrogen on brain tissues mediating lordosis. Physiol. Behav. 32, 483-488, 1984.
Wilcox, J.N., Spelsberg, T.C., and Feder, H.H. Lack of an effect of estrogen on endogenous RNA polymerase II in guinea pig brain. J. Steroid Biochem. 21, 513-518, 1984.
Roberts, J.L., Gee, C.E., Wilcox, J.N., and Blum, M. Analysis of neuropeptide gene expression in discrete neural regions and individual neurons. Society for Neuroscience 1983 Short Course, Molecular Approaches to the Nervous System, Nov. 1983.
Roberts, J.L., Wilcox, J.N., and Gee, C.E. In situ cDNA:mRNA hybridization a technique to study gene expression in individual cells. In: Endocrinology, Proceedings of the 7th International Congress of Endocrinology, Quebec City, 1-7 July 1984 (F. Labrie & L. Proulz, eds.), Excerpta Medica, Amsterdam, 1984, pp. 1117-1120.
Wilcox, J.N. and Roberts, J.L. Estrogen decreases rat hypothalamic proopiomelanocortin mRNA levels. Endocrinology 117, 2392-2396, 1985.
Wilcox, J.N. Analysis of steroid action on gene expression in the brain. In: Reproduction a behavioral and neuroendocrine perspective (B.R. Komisaruk, H.I. Siegel, M.F. Cheng, and H.H. Feder, eds.), Ann. N.Y. Acad. Sci. 474, 453-460, 1986
Wilcox, J.N., Gee, C.E., and Roberts, J.L. In situ cDNA:mRNA hybridization: Development of a technique to measure mRNA levels in individual cells. In: Methods in Enzymology, Vol 124, Neuroendocrine Peptides (P.M. Conn, ed.), Academic Press, pp510-533, 1986.
Roberts, J.L. and Wilcox, J.N. Analysis of specific mRNAs in individual cells. In: Immunocytochemistry, practical applications in pathology and biology, 2nd Ed. (J.M. Polak and S. Van Noorden, ed.) John Wrights and Sons, London, pp198-204, 1986.
Roberts, J.L., Wilcox, J.N., and Blum, M. The regulation of proopiomelanocortin gene expression by estrogen in the rat hypothalamus. In: A. Guiditta, B.B. Kaplan and C. Zomzely-Neurath (Eds.), Role of RNA and DNA Activity in Brain Function, Martinus Nijhof Co., Boston, 1986, pp.112-122.
Herbert, J., Wilcox, J.N. Pham, K-T.C., Fremeau, R.T., Zeviani, M., Dwork, A., Soprano, D.R., Makover, A., Goodmen, D.S., Zimmerman, E.A., Roberts, J.L., and Schon, E.A. Transthyretin: A choroid plexus-specific transport protein in human brain. Neurology 36, 900-911, 1986.
Wilcox, J.N., Roberts, J.L., Chronwall, B.M., Bishop, J.F., and O'Donohue, T. Localization of proopiomelanocortin mRNA in functional subsets of neurons defined by their axonal projections. J. Neurosci. Res. 16, 89-96, 1986,
Fremeau, R.T., Lundblad, J.R., Pritchett, D.B., Wilcox, J.N., and Roberts, J.L. Regulation of pro-opiomelanocortin gene transcription in individual cell nuclei. Science 234, 1265-1269, 1986.
Mason, A.J., Pitts, S.L., Nikolics, K., Szonyi, E., Wilcox, J.N., Seeburg, P.H., and Stewart, T.A. The hypogonadal mouse: Reproductive functions restored by gene therapy. Science 234, 1372-1378, 1986.
Mason, A.J., Pitts, S.L., Nikolics, K., Szonyi, E., Wilcox, J.N., Seeburg, P.H., and Stewart, T.A. Gonadal development and gametogenesis in the hypogonadal mouse are restored by gene transfer. Ann. N.Y. Acad. Sci. 513,16-26, 1987.
Coffey, R.J., Derynck, R., Wilcox, J.N., Bringman, T.S., Goustin, A.S., Moses, H.L., and Pittelkow, M. Growth factor induced expression of transforming growth factor alpha in normal human keratinocytes. Nature 328, 817-820, 1987.
Rosenthal, A., Chan, S.Y., Henzel, W., Haskell, C., Kuang, W-J., Chen, E., Wilcox, J.N., Ullrich, A., Goedel, D.V., and Routtenberg, A. Primary structure and mRNA localization of protein F1, a growth-related protein kinase C substrate, associated with synaptic plasticity. EMBO J. 6,3641-3646, 1987.
Wilcox, J.N. and Derynck, R. Localization of cells synthesizing transforming growth factor alpha mRNA in the mouse brain. J. Neuroscience 8,1901-1904, 1988.
Wilcox, J.N. and Derynck, R. Developmental expression of transforming growth factors alpha and beta in mouse fetus. Molec. Cell. Biol. 8,3415-3422, 1988.
Wilcox, J.N., Smith, K.S., Williams, L.T., Schwartz, S., and Gordon, D. Platelet-derived growth factor mRNA detection in human atherosclerotic plaques by in situ hybridization. J. Clin. Invest. 82, 1134-1143, 1988.
Alho, H., Fremeau, R.T.,Tiedge,H, Wilcox, J.N., Bovolin, P., Brosius, J., Roberts, J.L., Costa, E. Diazepam binding inhibitor gene expression: Location in brain and peripheral tissues of rat. Proc. Natl. Acad. Sci. 85, 7018-7022, 1988
Harris, N.S., Wilcox, J.N., Dutlow, C.M., Flanagan, C.A., Prescott, R.A., Roberts, J.L., Eidne, K.A. and Millar, R.P. A putative autocrine role for GnRH in mammary carcinoma cell lines. Prog. Cancer Res Therapy, Vol. 35: Hormones and Cancer 3, ed by F. Bresciani, RJB King, ME Lipprian and JP Raynaud, Raven Press, New York 1988, pp174-178.
Pollard, H., Wilcox, J.N., Malfroy, B., Runco, P., Verroust, P. and Schwartz, J.C. Localization of enkephalinase (EC 3.4.24.11) in rat brain: Radioimmunohistochemical and mRNA hybridization data. Proceedings of Int. Neuro. Res Conf. 1988.
Schofield, P.R., McFarland, K.C., Hayflick, J.S., Wilcox, J.N., Cho, T.M., Roy, S., Lee, N.M., Loh, H.H., and Seeburg, P.H. Molecular characterization of a new immunoglobulin superfamily protein with potential roles in opiod binding and cell contact. EMBO J. 8, 489-495, 1989.
Wilcox, J.N., Smith, K.M., Schwartz, S.M., Gordon, D. Localization of tissue factor in the normal vessel wall and in the atherosclerotic plaque. Proc Natl. Acad.Sci. 86, 2839-2843, 1989.
Harvey, R.A., Kim, H.C., Pincus, J., Trooskin, S.Z., Wilcox, J.N.,
Greco, R.S. Binding of tissue plasminogen activator to vascular grafts. Thromb. Haemostasis 61, 131-136, 1989.
Gordon, D., Schwartz, S.M., Benditt, E.P., and Wilcox, J.N. Growth factors and cell proliferation in human atherosclerosis. Transplant. Proc. 21, 3692-3694, 1989.
Wilcox, J.N., Pollard, H. Moreau, J., Schwartz, J.C., Malfroy, B. Localization of enkephalinase mRNA in rat brain by in situ hybridization: Comparison with immunohistochemical localization of the protein. Neuropeptides 14, 77-83, 1989.
Fremeau, R.T., Autelitano, D.J., Blum, M., Wilcox, J.N. and Roberts, J.L. Intervening sequence-specific in situ hybridization: Detection of the pro-opiomelanocortin gene primary transcript in individual neurons. Molecular Brain Research, 6, 197-201, 1989.
Derynck, R., Lindquist, P.B., Bringman, T.S., Wilcox, J.N., Elder, J.T., Fisher, G.L., Voorhees, J.J., Moses, H.L., Pittelkow, M.R., and Coffey, R.J. Expression of transforming growth factor alpha gene in tumor cells and normal cells. In: Cancer Cells: Molecular Diagnostics of Human Cancer, Vol. 7, Ed. by M. Furth and M. Greaves, Cold Spring Harbor Press, Cold Spring Harbor, pp297-301, 1989.
Wilcox, J.N., Schwartz, S.M., and Gordon, D. Local production of PDGF in the human atherosclerotic plaque. In: Growth factors, differentiation factors and cytokines, ed. by A. Habenicht, Springer-Verlag, Heidelberg, 1990.
Smith, K.M., Lawn, R.M., and Wilcox, J.N. Cellular localization of apolipoprotein D and lecithin:cholesterol acyl transferase mRNA in rhesus monkey tissues by in situ hybridization. J. Lipid Res. 31, 995-1004, 1990.
Schwall, R.H., Mason, A.J., Wilcox, J.N., Bassett, S. A. and Zeleznik, A.J. Localization of inhibin/activin subunit mRNAs within the primate ovary. Molec. Endocrinol. 4, 75-79, 1990.
Wilcox, J.N. Analysis of local gene expression in human atherosclerotic plaques by in situ hybridization. Trends in Cardiovascular Med. 1, 17-24, 1990.
Fava, R.A., Casey, T.T., Wilcox, J.N., Pelton, R.W., Moses, H.L., and Nanney, L.B. Synthesis of transforming growth factor-b1 by megakaryocytes and its localization to megakaryocyte and platlet a-granules. Blood 76, 1946-1955, 1990.
Majesky, M.W., Reidy, M.A., Bowen-Pope, D.F., Hart, C.E., Wilcox, J.N., and Schwartz, S.M. PDGF ligand and receptor gene expression during repair of arterial injury. J. Cell Biol. 111, 2149-2158, 1990.
Golden, M.A., Au, Y.P.T., Kirkman, T.R., Wilcox, J.N., Raines, E.W., Ross, R., and Clowes, A.W. Platelet derived growth factor activity and mRNA expression in healing vascular grafts in baboons. The association in vivo of PDGF mRNA and protein with cellular proliferation. J. Clin. Invest. 87, 406-414, 1991.
Wilcox, J.N. Thrombin and other potential mechanisms underlying restenosis. Circulation 84, 432-435, 1991.
Wilcox, J.N., Augustine, A., Goedell, D.V., and Lowe, D.G. Differential regional localization of three natriuretic peptide receptor genes within primate tissues. Molec. Cell. Biol 11, 3454-3462, 1991.
Nelken, N., Coughlin, S.R., Gordon, D., and Wilcox, J.N. Monocyte chemoattractant protein-1 in human atheromatous plaques. J. Clin. Invest. 88, 1121-1127, 1991. (Recipient of the Henry Christian Memorial Award from the AFCR 1991.)
Wilcox, J.N. Analysis of local gene expression in human atherosclerotic plaques. J. Vascular Surg. 15, 913-916, 1992.
Jang, I-K, Gold, H.K., Leinback, R.C., Fallon, J.T., Collen, D., and Wilcox, J.N. Antithrombotic effect of a monoclonal antibody against tissue factor in a rabbit model of platelet-mediated arterial thrombosis. Arteriosclerosis andThrombosis 12, 948-954, 1992.
Marsden, P.A., Schappert, K.T., Chen, H.S., Flowers, M., Lamas, S., Sundell, C.L., Wilcox, J.N., and Michel, T. Molecular cloning and characterization of human endothelial nitric oxide synthase. FEBS Lett. 307, 287-293, 1992.
Wilcox, J.N. The molecular mechanisms controlling smooth muscle proliferation in the arterial wall. Therapeutic Res. 13, 4408-4430, 1992.
Langford, K.G., Zhou, Y., Russell, L.D., Wilcox, J.N., and Bernstein, K.E. Regulated expression of testis angiotensin-converting enzyme during spermatogenesis. Biol. Reprod. 48, 1210-1218, 1993.
Martinez-Maldonado, M., Benabe, J.E., Wilcox, J.N., Wang, S., and Luo, C. Renal renin, angiotensinogen, and ANG I-converting enzyme gene expression: influence of dietary protein. Am. J. Physiol. 264, F981-F988, 1993.
Wilcox, J.N. Molecular Biology - Insight into the causes and prevention of restenosis after arterial intervention. Am. J. Cardiol. 72, E88-E95, 1993.
Wilcox, J. Fundamental principles of in situ hybridization. J. Histochem. Cytochem. 41, 1725-1733, 1993.
Lobie, P.E., Garcia-Aragon, J., Lincoln, D.T., Bernard, R. Wilcox, J.N., and Waters, M.J. Localization and ontogeny of growth hormone receptor gene expressionin the central nervous system. Dev. Brain Res. 74, 225-233, 1993.
Kraiss, L.W., Raines, E.W., Wilcox, J.N., Seifert, R.A., Barrett, T.B., Kirkman, T.R., Hart, C.E., Bowen-Pope, D.F., Ross, R., and Clowes, A.W. Regional expression of the platelet-derived growth factor and its receptors in a primate graft model of vessel wall assembly. J. Clin. Invest. 92, 338-348, 1993.
Pimentel, J.L., Martinez-Maldoado, M., Wilcox, J.N., Wang, S., and Luo, C. Regulation of renin-angiotensin system in unilateral ureteral obstruction. Kidney Int. 44, 390-400, 1993.
Benabe, J.E., Wang, S., Wilcox, J.N., and Martinez-Maldonado, M. Modulation of ANG II receptor and its mRNA in the normal rat by low protein feeding. Am. J. Physiol. 265, F660-F669, 1993.
Wilcox, J.N. and Harker, L.A. Molecular and cellular mechanisms of atherogenesis: Studies of human lesions linked with animal modeling. In Haemostasis and Thrombosis, 3rd ed., A.L. Bloom, C.D. Forbes, D.P. Thomas, and E.G.D. Tuddenham (eds), Chapter 49, pp1139-1152, Churchill Livingstone, Edinburgh, 1994.
Harker, L.A., Wilcox, J.N., Runge, M.S., Kelly, A.B., Lumsden, A.B., and Hanson, S.R. The role of thrombin in vascular lesion formation in nonhuman primates. Therapeutic strategies in vascular lesion formation. J. Vascular Surg. 20, 122-123, 1994.
Wilcox, J.N. Thrombotic mechanisms in atherosclerosis. Coronary Art. Dis. 5, 223-229, 1994.
Wilcox, J.N., Rodriguez, J., Subramanian, R., Ollerenshaw, J., Zhong, C., Hayzer, D.J., Horaist, C., Hanson, S.R., Lumsden, A., Salam, T., Kelly, A.B., Harker, L.A., and Runge, M. Characterization of thrombin receptor expression during vascular lesion formation. Circ. Res. 75, 1029-1038, 1994.
Wilcox, J.N. and Blumenthal, B.F. Thrombotic mechanisms in atherosclerosis: Potential impact of soy proteins. J. Nutr. 125, 631S-638S, 1995.
Wilcox, J.N., Nelken, N.A., Coughlin, S.R., Gordon, D., and Schall, T.J. Local expression of inflammatory cytokines in human atherosclerotic plaques. J. Athero. Thromb. 1, S10-S13, 1995.
Ohara, Y., Peterson, T.E., Sayegh, H.S., Subramanian, R.R., Wilcox, J.N., Harrison, D.G. Dietary correction of hypercholesterolemia in the rabbit normalizes endothelial superoxide anion production. Circulation 92, 898-903, 1995.
Gladson, C.L., Wilcox, J.N., Sanders, L., Gillespie, G.Y., Cheresh, D.A. Cerebral microenvironment influences expression of the vitronectin gene in astrocytic tumors. J. Cell. Sci. 108, 947-956, 1995.
Harker, L.A., Hanson, S.R., Wilcox, J.N., Kelly, A.B. Antithrombotic and antilesion benefits without hemorrhagic risks by inhibiting tissue factor pathway. Haemostasis 26, 76-82, 1996.
Scott, N.A., Cipolla, G., Ross, C., Martin, F., Simonet, L., Wilcox, J.N. Role of the adventita in vascular lesion formation after balloon overstretch injury of porcine coronary arteries. Circulation 93, 2178-2187, 1996
Sands, J.M., Naruse, M., Jacobs, J.D., Wilcox, J.N., Klein, J.D. Changes in aquaporin-2 protein contribute to the urine concentrating defect in rats fed a low-protein diet. J. Clin. Invest. 97, 2807-2814, 1996.
Wilcox, J.N., Scott, N.A. Potential role of the adventitia in arteritis and atherosclerosis. Int. J. Cardiol. 54, S21-S35, 1996.
Wilcox, J.N., Waksman, R., King, S.B., Scott, N.A. The role of the adventitia in the arterial response to angioplasty: The effect of intravascular beta irradiation. Int. J. Radiation Oncology Biol. Phys. 36, 789-796, 1996.
Wilcox, J.N., Waksman, R., King, S.B., Scott, N.A. Role of the adventitia in the arterial response to angioplasty. In: Vascular Brachytherapy, Waksman, R., King, SB, Crocker, IR, Mould, RF (eds), pp. 17-29, Nucletron-Oldelft, Waardgleder, Netherlands, 1996.
Fukui, T., Ishizaka, N., Rajagopalan, S., Laursen, J.B., Capers, Q., Taylor, W.R., Harrison, D.G., De Leon, H., Wilcox, J.N., Griendling, K.K. p22phox mRNA expression and NADPH oxidase activity are increased in aortas from hypertensive rats. Circ. Res. 80, 45-51, 1997.
DeLeon, H., Scott, N.A., Martin, F., Simonet, L., Bernstein, K.E., Wilcox, J.N. Expression of nonmuscle myosin heavy chain-B isoform in the vessel wall of porcine coronary arteries after balloon angioplasty. Circ. Res. 80, 514-519, 1997.
Wang, Y., Goligorsky, M., Lin, M., Wilcox, J.N., Marsden, P.A. A novel, testis-specific mRNA transcript encoding an NH2-terminal truncated nitric oxide synthase. J. Biol. Chem. 272, 11392-11401, 1997
Oyesiku, N.M., Wilcox, J.N., Wigston, D.J. Changes in expression of ciliary neurotrophic factor (CNTF) and CNTF-receptor alpha after spinal cord injury. J. Neurobiology 32, 251-261, 1997.
Kim, J.G., Sabbagh, F., Santanam, N., Wilcox, J.N., Medofrd, R.M., Parthasarathy, S. Generation and characterization of a polyclonal antibody against lipid peroxide-modified proteins. Free Radicals in Biol. and Med., 23, 251-259, 1997.
Ishizaka, N., De Leon, H., Laursen, J.B., Fukui, T., Wilcox, J.N., De Keulenaer, G.D., Griendling, K.K., Alexander, R.W. Angiotensin II-induced hypertension increases heme oxygenase-1 expression in rat aorta. Circulation 96, 1923-1929, 1997
Waksman, R., Rodriguez, J.C., Robinson, K.A., Cipolla, G.D., Crocker, I.R., Scott, N.A., King, S.B., Wilcox, J.N. Effect of intravascular irradiation on cell proliferation, apoptosis and vascular remodeling after balloon overstretch injury of porcine coronary arteries. Circulation 96, 1944-1952, 1997.
Capers, Q., Alexander, R.W., Lou, P., DeLeon, H., Wilcox, J.N., Howard, A.B., Taylor, W.R. Monocyte chemoattractant protein-1 expression in aortic tissues of hypertensive rats. J. Hypertension 30, 1397-1402, 1997.
Wilcox, J.N., Subramanian, R., Sundell, C., Pollock, J., Harrison, D., and Marsden, P. Expression of nitric oxide synthase in normal and atherosclerotic vessels. Arterio. Thromb. Vas. Biol. 17, 2479-2488, 1997.
Rock, B.M., Xin, L., Wilcox, J.N. Two Gq class G proteins are expressed in human keratinocytes. J. Invest. Dermatol. 109, 645-549, 1997.
Shoji, M., Hancock, W.W., Abe, K., Micko, C., Casper, K.A., Baine, R.M., Wilcox, J.N., Danave, I., Dillehay, D.L., Matthews, E., Contrino, J., Morrissey, J.H., Gordon, S., Edgington, T.S., Kudryk, B., Kreutzer, D.L., and Rickles, F.R. Activation of coagulation and angiogenesis in cancer: Immunohistochemical localization in situ of clotting proteins and vascular endothelial growth factor in human cancer. Am. J. Pathol. 152, 399-411, 1998.
Noguchi, S., Numano, F., Gravanis, M.B., Wilcox, J.N. Increased levels of soluble forms of VCAM-1 and ICAM-1 in the plasma of patients with Takayasu arteritis. Int. J. Cardiol. 66, S23-S33, 1998.
Scott, N.A. and Wilcox, J.N. The role of adventitial vasculature in restenosis: a new view of an old problem. J. Am. Coll. Cardiol. 32, 2072-2079, 1998.
Huraux, C., Makita, T., Kurz, S., Yamaguchi, K., Szlam, F., Tarpey, M.M., Wilcox, J.N., Harrison, D.G., Levy, J.H. Superoxide production, risk factors and endothelium-dependent relaxations in human internal mammary arteries. Circulation 99, 53-59, 1999.
Giese, N.A, Marijianowski, M.M.H., McCook, O., Hancock, A., Ramakrishnan, V., Fretto, L.J., Koziol, J.A., Wilcox, J.N., Hanson, S.R. The role of alpha and beta platelet-derived growth factor receptor in the vascular response to injury in non-human primates. Arterio. Thromb. Vas. Biol. 19, 900-909, 1999.
Wang. N.P., Bufkin, B.L., Nakamura, M., Zhao, Z.Q., Wilcox, J.N., Hewan-Lowe, K.O., Guyton, R.A., Vinten-Johansen, J. Ischemic preconditioning reduces neutrophil accumulation and myocardial apoptosis. Ann. Thorac. Surg. 67, 1689-1695, 1999.
Wang, Y., Newton, D.C., Robb, G.B., Kau, C-L, Miller, T.L., Cheung, A.H., Hall, A.V., VanDamme, S., Wilcox, J.N., Marsden, P.A. RNA diversity has profound effects on the translation of neuronal nitric oxide synthase. Proc. Natl. Acad. Sci. USA 96, 12150-12155, 1999.
Zhao, ZQ, Nakamura, M., Wang, N-P, Wilcox, J.N., Shearer, S., Guyton, R.A., Vinten-Johansen, J. Adenosine during the early phase of reperfusion reduces injury of vascular endothelium and death of myocytes. Coronary Art. Dis. 10, 617-628, 1999.
Naik, S.M., Cannon, G., Burbach, G.J., Singh, S.R., Swerlick, R.A., Wilcox, J.N., Ansel, J.C., Caughman, S.W. Human keratinocytes constituitively express interleukin-18 and secrete biologically active interleukin-18 after treatment with pro-inflammatory mediators and dinitrochlorobenzene. J. Invest. Dermatol. 113, 766-772, 1999.
Nakamura, M., Wang, N-P, Zhao, A-Q, Wilcox, J.N., Thourani, V., Guyton, R.A., Vinten-Johansen, J. Preconditioning decreases Bax expression, PMN accumulation and apoptosis in reperfused rat heart. Cardiovas. Res. 45, 661-670, 2000.
Zhao, Z-Q, Nakamura, M., Wang, N-P, Wilcox, J.N., Shearer, S., Ronson, R.S., Guyton, R.A., Vinten-Johansen, J. Reperfusion induces myocardial apoptotic cell death. Cardiovas. Res. 45, 651-660, 2000.
Bush, E., Maeda, N., Kuziel, W.A., Dawson, T.C., Wilcox, J.N., DeLeon, H., Hicks, M., Alexander, R.W., Taylor, W.R. CC chemokine receptor 2 is required for macrophage infiltration and vascular hypertrophy in an angiotensin II induced hypertension. Hypertension 36, 360-363, 2000.
Hoshino, Y., Kurabayashi, M., Kanda, T., Hasegawa, A., Sakamoto, H., Okamoto, E., Kowase, K., Watanabe, N., Manabe, I., Suzuki, T., Nakano, A., Takase, S., Wilcox, J.N., Nagai, R. Regulated Expression of the BTEB2 transcription factor in injured vascular smooth muscle cells: Analysis of developmental and pathological expression profiles shows implications as a predictive factor for restenosis. Circulation 102, 2528-2534, 2000.
Wallner, K., Sharifi, B., Shah, P.K., DeLeon, H., Wilcox, J.N. Expresion of tenascin mRNA by adventitial myofibroblasts during vascular remodeling after angioplasty. J. Am. Coll. Cardiol. 37, 655-661, 2001.
Lawn, R.M., Wade, D.P., Couse, T.L., Wilcox, J.N. Localization of Human ATP-binding cassette transporter 1 (ABC1) in normal and atherosclerotic tissues. Arterio. Thromb. Vas. Biol. 21, 378-385, 2001.
Manuscripts submitted:
Ziegler, T.R., Estivariz, C.F., Gu, L.H., Umeakunne, K., Wallace, T.M., Diaz, E.E., Galloway, J.R., Wilcox, J.N., Pascal, R.R., Leader, L.M. Expression of oligopeptide transporter PepT1 messenger RNA in human gut mucosa and colonic upregulation in short bowel syndrome. In press Gastroenterology.
Shibahara, T., Wilcox, J.N., Couse, T., Madara, J.L. Characterization of epithelial chemoattractants for intestinal intraepithelial lymphocytes in humans. In Press Gastroenterology.
Noguchi, S., Ezban, M., Wilcox, J.N. Localization of tissue factor, factor VII and factor X in normal and atherosclerotic vessels. In review Arterio. Thromb. Vas. Biol.
Scott, N.A., Crocker, I.R., Yin, Q.Q., Sorescu, D., Wilcox, J.N., Griendling, K.K. Inhibition of vascular cell growth by X-ray irradiation: Comparison with gamma radiation and mechanism of action. In review Circulation.
Okamoto, E., Couse, T., De Leon, H., Vinten-Johansen, J., Goodman, R.B., Scott, N.A., Wilcox, J.N. Perivascular inflammation after balloon angioplasty of porcine coronary arteries. In review Circulation.