Incubate slides, 55°C, 30 min.
Xylenes, 2 times, 2 min. each
100% EtOH, 2 times, 2 min. each
95% EtOH, 2 times, 2 min. each
80% EtOH, 2 min.
75% EtOH, 2 min.
50% EtOH, 2 min.
A. Remove 100µl from Tube 2 (Label solution) for 2 negative controls (50µl each). Do this even if you are omitting this negative control so that volumes and concentrations will remain consistent for the labeling.
B. Add Total volume (50µl,) of Tube 1 (TdT) + remainder of Tube 2 (450µl).
5ml 100mM Tris, pH 8.2
1 drop Levamisole
2 drops each of Solution 1, 2, and 3 of either Vector substrate